Seasonal variability of water parameters and construction of laccase biosensors using ureasil polymers for analysis of water pollutions

T.S. Kavetskyy; O.M. Demkiv; A.M. Pryima; N.K. Hoivanovych; I.V. Bryndzia; I.V. Briukhovetska; I.F. Drozd; S.V. Voloshyn; G.M. Kossak; V.M. Senkiv; O.I. Mykytchyn; G.V. Krechkivska; L.M. Kropyvnytska; S.Y. Voloshanska; A.E. Kiv

doi:10.15330/pcss.25.3.461-470

Authors

T.S. Kavetskyy Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine; Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovakia; South Ukrainian National Pedagogical University named after K.D. Ushynsky, Odesa, Ukraine
O.M. Demkiv Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland
A.M. Pryima Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine; Lviv State University of Life Safety, Lviv, Ukraine
N.K. Hoivanovych Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
I.V. Bryndzia Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
I.V. Briukhovetska Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
I.F. Drozd Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
S.V. Voloshyn Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
G.M. Kossak Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
V.M. Senkiv Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
O.I. Mykytchyn Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
G.V. Krechkivska Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
L.M. Kropyvnytska Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
S.Y. Voloshanska Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
A.E. Kiv South Ukrainian National Pedagogical University named after K.D. Ushynsky, Odesa, Ukraine; Ben-Gurion University of the Negev, Beer-Sheva, Israel

DOI:

https://doi.org/10.15330/pcss.25.3.461-470

Keywords:

underground water, organoleptic and physico-chemical indicators, polymers, positron annihilation, free volume, crosslinking, laccase, biosensors, water pollution

Abstract

Physical and chemical indicators of underground waters of the Skole region were studied. The samples were taken from natural water sources of Skole district of Lviv region and analyzed within organoleptic and physico-chemical indicators of the quality of water. The organoleptic and physico-chemical indicators of the quality of natural water sources of the studied area meet the requirements of the State sanitary norms and rules. Organic-inorganic ureasil polymers with different precursors were used as holding matrixes in construction of laccase biosensors for analysis of water pollution. Cyclic voltammetry and chronoamperometric measurements were carried out to estimate the main parameters of the constructed biosensors. It is found that the sensitivity of the constructed biosensors correlates well with the network properties (free volume and crosslinking) of the investigated polymers studied using positron annihilation lifetime spectroscopy and swelling measurements. The same correlation was also earlier reported for ureasil composites of different prehistory and photopolymers and it seems to be universal that allows controlled biosensing according to the required needs of analysis.

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